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KMID : 1094720230280010125
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Biotechnology and Bioprocess Engineering
2023 Volume.28 No. 1 p.125 ~ p.136
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Hsa_circ_0001165 Regulates the Malignant Phenotype and Angiogenesis of Prostate Cancer Cells through miR-654-3p/DDAH1
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Shiben Ji
Ci Zhang
Yang Xiang
Guohong Yin
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Abstract
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Prostate cancer (PCa) is one of the most common malignant tumors, with a high rate of metastasis and recurrence. Circular RNA_0001165 (circ_0001165) has been shown to be involved in the advance of PCa. However, the interaction between circ_0001165 and microRNA in PCa has not been studied. Quantitative real-time polymerase chain reaction was used to detect the expression of related genes in PCa tissues and cells. The expression of related epithelial-mesenchymal transition proteins was detected by Western blotting. The interaction of miR-654-3p with circ_0001165 or dimethylarginine dimethylaminohydrolase 1 (DDAH1) has been notarized by Dual-luciferase reporter assay and RNA immunoprecipitation assay. Xenotransplantation experiments confirmed the function of circ_0001165 in vivo. Circ_0001165 and DDAH1 are significantly high-expressed in PCa tissues and cells. Silencing circ_0001165 can reduce the proliferation, migration, invasion and tube formation of LNCaP and DU145 cells. MiR-654-3p is a target of circ_0001165. Silencing circ_0001165 can inhibit the malignant behavior of PCa cells by releasing miR-654-3p. In addition, DDAH1 is a target of miR-654-3p. Overexpression of DDAH1 partially restored the inhibitory effect of miR-654-3p on cell proliferation. Animal experiments confirmed the anti-tumor effect of silence circ_0001165 in vivo. Circ_0001165 regulates the expression of DDAH1 by regulating miR-654-3p, thereby mediating the process of PCa, and at least partially promoting the development of PCa cells, providing a novel targeted therapy for PCa.
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KEYWORD
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circ_0001165, miR-654-3p, DDAH1, prostate cancer
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